Toxo IgG/IgM Antibody Rapid Test Cassette is based on sandwich lateral flow immunochromatographic assay. The test cassette has a testing window. The testing window has two invisible Test zones (IgG/IgM) and C (control) zone. When sample is applied into the sample well on the cassette, the liquid will laterally flow on the surface of the test strip. If there is enough Toxoplasma IgG/IgM antibody in the sample, a visible line will appear in IgG/IgM zone. The C line should always appear after a sample is applied, indicating a valid result. By this means, the cassette can accurately indicate the presence of Toxoplasma IgG/IgM antibody in the sample.
- Collect fresh whole blood, or separate serum or plasma from blood as soon as possible to avoid hemolysis. Only use clear, non-hemolyzed specimens. Do not leave the specimens at room temperature for prolonged periods. Serum and plasma specimens may be stored at 2-8 °C for up to 3 days. For long term storage, serum and plasma specimens should be kept below -20°C.
- Place the test cassette on a clean and level surface. Hold the dropper vertically and Transfer 1 drop of sample (approximately 40 μl) and immediately add 3 drops of buffer (approximately 80 μl) into the specimen well (S), then start the timer. See illustration above.
- Read the result in 5-10 minutes. Do not interpret results after 15 minutes